Pierce Silver Stain uses formaldehyde in the stain and developer working solutions. However, the procedure accompanying the kit for mass spectrometry is optimized to maximize polypeptide recovery without greatly sacrificing sensitivity. Thermo Scientific Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and sensitive silver staining of proteins in polyacrylamide gels and efficient. Related posts: Bioz Stars score: 99/100, based on 1 PubMed citations. described in this Tech Tip has been validated for use with the Pierce Silver Stain Kit. 2. Two assay procedures are presented. Pierce Silver Stain For Mass Spectrometry, supplied by Thermo Fisher, used in various techniques. Mass Spectrometry Kit, supplied by Thermo Fisher, used in various techniques. Home . In standard mini gels, proteins are detectable at greater than 0.25ng per band or spot. 1.Pierce Silver Stain Kit - Thermo Fisher Scientific; 2.Pierce Silver Stain for Mass Spectrometry - Thermo Fisher; 3.Thermo Scientific Pierce Silver Stain Kit Kit; 1L kit:Gel Electrophoresis; 4.Thermo Scientific Pierce Silver Stain for Mass Spectrometry Kit; 5.Pierce Color Silver Stain Kit, Thermo Scientific - VWR Get tips and helpful information related to the use of our protein stains for both in-gel and on-membrane detection, from choosing the right stain for your application to optimizing your staining procedure to get optimal band detection. For best results, use the Pierce Silver Stain Kit for Mass Spectrometry (Product No. Compare different protein staining methods Highlights of silver stains: Manufacturer: Thermo Scientific 24600 Catalog No. Decant water and add Fixing Solution to the gel. Get advice for use of the Pierce Power stainer for rapid protein gel staining. Decant water and add Fixing Solution to the gel. It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. Manufacturer: Thermo Scientific 0024600 Catalog No. Procedure 1. Thermo Scientific Pierce Silver Stain for Mass Spectrometry Reversibly stain proteins in polyacrylamide gels to identify bands to excise; then destain the gel pieces for analysis by mass spectrometry. Pierce Silver Stain for Mass Spectrometry. Protein Quantitation and Staining 36-42 Introduction 36 BCA Protein Assay 37 Micro BCA Assay 38 Silver Stain for Mass Spectrometry 39 Protein Stains 41 Protein Digestion 43-53 Introduction 43 Trypsin Protease, MS Grade 44 LysN Protease, MS Grade 46 Lys-C Protease, MS Grade 47 Asp-N Protease, MS Grade 47 Glu-C Protease, MS Grade 48 The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. Get All The Latest News Help & Support Center English Deutsch; Change Country; 0. Best practice is freeze at -80 > 3hr and then lyophilize. The protocol has been optimized for . Of these, the Test Tube Procedure requires a larger volume (0.1 ml) of protein sample; however . This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. Shop. 2. The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. Bioz Stars score: 99/100, based on 1 PubMed citations. Procedure 1. We offer several optimized silver staining kits depending on your research needs. Supplier: Thermo Scientific The Pierce Silver Stain Kit is a rapid, ultra-sensitive, and versatile silver stain system for protein detection in polyacrylamide gels, yielding consistent and reliable results. Pierce Silver Stain Kit Pierce Biotechnology PO Box 117 (815) 968 -0747 Thermofisher.com 3747 N. Meridian Road Rockford, lL 61105 USA (815) 968 -7316 fax 2 Additional Materials Required Ethanol, ~50mL Acetic Acid, ~25mL Ultrapure water, ~1L Procedure for Silver Staining Proteins in Polyacrylamide Gels Important Notes: Wash gel in ultrapure water for 5 minutes. PI24600 $344.50 / Each Qty Check Availability Add to cart Description Specifications Silver Stain for Mass Spectrometry Pierce Biotechnology PO Box 117 (815) 968 -0747 thermofisher.com 3747 N. Meridian Road Rockford, lL 611 05 USA (815) 968 -7316 fax 2 B. 5.Thermo Scientific Pierce Silver Stain for Mass Spectrometry Kit; 6.ProteoSilver Silver Stain Kit - Sigma-Aldrich; 7.Pierce Color Silver Stain Kit, Thermo Scientific - VWR; 8.Bielschowsky Silver Stain Kit (ab245877) - Abcam; 9.Silver Stains | Biocompare. Description: . Replace the water and wash for another 5 minutes. We offer several optimized silver staining kits depending on your research needs. For Research Use Only. Not for use in diagnostic procedures. (You should see no powder [detergent] or salt). Specifications Detection Location In-Gel Detection It is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background. English Deutsch; Change Country. ZERO BIAS - scores, article reviews, protocol conditions and more Silver protein stains is an ultra-sensitive method for monochromatic detection of proteins in 1D or 2D polyacrylamide gels with metallic silver ions (Ag). If mass spectrometry is the intended downstream application, choose one of our two silver stain kits optimized for peptide recovery after in-gel trypsin digestion for mass spectrometry. The Pierce Silver Stain Kit is a metallic silver (Ag) protein stain that yields a remarkably clear and uniform gel background while delivering consistent, high-sensitivity staining results at each and every use. If mass spectrometry is the intended downstream application, choose one of our two silver . ZERO BIAS - scores, article reviews, protocol conditions and more. The optimized staining method ensures extremely sensitive staining while minimizing covalent crosslinking of protein to the gel matrix, which can inhibit protein recovery. Silver Stains. Thermo Scientific Pierce Silver Stain for Mass Spectrometry Reversibly stain proteins in polyacrylamide gels to identify bands to excise; then destain the gel pieces for analysis by mass spectrometry. PI24600 $440.03 / Each Quantity Add to cart Description Specifications Documents Description Thermo Scientific Pierce Silver Stain for Mass Spectrometry is a complete kit for rapid and sensitive silver staining of proteins in polyacrylamide gels and efficient destaining of excised gel pieces for mass spectrometry analysis.Features Wash gel in ultrapure water for 5 minutes. Pierce Silver Stain for Mass Spectrometry, 1L kit from Thermo Scientific Pierce Protein Research Products Product Specs Item Pierce Silver Stain for Mass Spectrometry, 1L kit BCA Kit Protocol (pierce) standard that is similar in quality to the unknown; for example, a bovine gamma globulin (BGG) standard (see Related Pierce Products) may be used when assaying immunoglobulin samples. Replace the water and wash for another 5 minutes. No offer available . Silver Stain for Mass Spectrometry Pierce Biotechnology PO Box 117 (815) 968 -0747 thermofisher.com 3747 N. Meridian Road Rockford, lL 611 05 USA (815) 968 -7316 fax 2 B. 24600), which includes an optimized procedure and all the necessary reagents for staining gels and destaining gel pieces before in-gel trypsin digestion and MS. This kit enables both first-time and experienced users to achieve consistent and reliable staining using high-, low-, and gradient-percentage gels in 1D and 2D formats. Extract the gel by adding 50 l 0.1% TFA, 60% CH3CN and shaking at room temperature for 60 minutes (use more volume if necessary) Repeat steps 8 and 9, twice and Combine extracts with the supernatant from step 8.
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